complete protease inhibitor cocktail  (Thermo Fisher)

Journal: bioRxiv

Article Title: Interdigitating coiled-coil tetramers define the helical architecture of GFAP filaments in astrocytes

doi: 10.64898/2026.02.23.707361

Figure Lengend Snippet: A: Complete model of VIM filaments. VIM elongates through extension of compact octameric protofibrils running parallel to the helical axis. Protofibrils are linked laterally through the tail domains and centrally through the head domains. Right: lattice representation of VIM assembly. B: Complete model of the GFAP filament. GFAP elongates through extension of tetrameric protofilaments, which laterally associate to form octameric protofibrils. Protofibrils laterally associate to form the filament surface and are linked centrally through the head domains. Tail domains are largely extruded from the filament surface. Right: lattice representation of GFAP assembly. Note: Each rectangular component in schematics represents a parallel dimer.

Article Snippet: In brief, bacterial pellets were sonicated in a buffer containing 50 mM Tris HCl (pH 8), 200 mM NaCl, 25% glycerol, 1 mM EDTA, 10 mg/ml lysozyme, 20 mM MgCl 2 , 8 μg/ml DNase 1, 40 μg/ml RNase A, 1% NP40, 1% deoxycholic acid and one cOmplete Protease Inhibitor Cocktail tablet (Merck, 5056489001) centrifuged at 12,000g for 30 minutes at 4 °C.

Techniques: